Mouse anti Human CD7, conjugated with FITC

Catalogue Number: 0072-EXA

Manufacturer:ExAlpha
Type:Monoclonal Primary Antibody - Conjugated
Alias:T-cell antigen CD7; GP40; T-cell leukemia antigen; TP41; Leu-9
Shipping Condition:Blue Ice
Unit(s): 100 tests
Host name: Mouse
Clone: MG34
Isotype: IgG2a
Immunogen: CD7=Derived from the hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells of BALB/c mice immunized with T-acute lymphoblastic leukemia (T-ALL) cells.
Application: FC

Description

Description: Identification of human T lymphocytes in multiple stages of T cell development, including a major subset of mature peripheral T cells. CD7 antigen is often increased on T leukemic cells. The CD7 molecule is a 40,000 M.W. surface antigen that is expressed on T-Lymphoid and myeloid precursors in fetal liver and bone marrow.

Additional Text

Gene ID

924

Gene Name

CD7

Uniprot ID

P09564

Purification

Protein A/G Purified

Antibody Clonality

Monoclonal

Application Notes

PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8 C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Storage Note

Product should be stored at 4-8C. DO NOT FREEZE