Mouse anti Human CD13, conjugated with FITC

Catalogue Number: 0132-EXA

Manufacturer:ExAlpha
Type:Monoclonal Primary Antibody - Conjugated
Alias:CD13 FITC
Shipping Condition:Blue Ice
Unit(s): 100 tests
Host name: Mouse
Clone: E735
Isotype: IgG1
Immunogen: CD13=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c x C57BL16 hybrid mice immunized with KG-1a cell line.
Application: FC

Description

Description: Identification of human Monocytes and Granulocytes expressing the 150 kD M.W. surface antigen. CD13 is a membrane enzyme, Aminopeptidase N. Myeloid cells in regenerating bone marrow will have an increased expression of CD13 in comparison with normal bone marrow myeloid cells.

Additional Text

Gene ID

290

Gene Name

ANPEP

Uniprot ID

P15144

Purification

Protein A/G Purified

Antibody Clonality

Monoclonal

Application Notes

PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20C). Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Storage Note

Product should be stored at 4-8C. DO NOT FREEZE