Mouse anti Human CD14, conjugated with FITC
Catalogue Number: 0142-EXA
| Manufacturer: | ExAlpha |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Alias: | CD14 FITC |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 tests |
| Host name: | Mouse |
| Clone: | FWKW |
| Isotype: | IgG2b |
| Immunogen: | |
| Application: | FC |
Description
Description: Identification of human Monocyte/Macrophage cells expressing the 55 kDa M.W. surface antigen. CD14 is present on 70-93% of Monocyte/Macrophage cells in normal peripheral blood.
Additional Text
Gene Name
CD14
Gene ID
929
Purification
Protein A/G Purified
Uniprot ID
P08571
Antibody Clonality
Monoclonal
Application Notes
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.
Storage Note
Product should be stored at 4-8C. DO NOT FREEZE
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