Mouse anti Human CD15
Catalogue Number: 0151-EXA
| Manufacturer: | ExAlpha |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | FUT4 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | ARE |
| Isotype: | IgM |
| Immunogen: | |
| Application: | FC |
Description
Description: Identification of CD15 that recognizes a human mylelomonocytic antigen. The structure recognized by CD15 antibodies is lacto-N-fucopentose III.1. The CD15 antigen is present on greater than 95% of mature peripheral blood eosinophils and neutrophils. It is present at low density on circulating monocytes. In lymphoid tissue CD15 reacts with Reed-Sternberg cells of Hodgkins disease and with granulocytes. However, CD15 reacts with only a few tissue macrophages and does not react with dendritic cells.
Additional Text
Gene ID
2526
Gene Name
FUT4
Purification
Protein A/G Purified
Uniprot ID
P22083
Antibody Clonality
Monoclonal
Application Notes
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Storage Note
Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles
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