Mouse anti Human CD16, conjugated with Biotin
Catalogue Number: 0163-EXA
| Manufacturer: | ExAlpha |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Alias: | CD16 Biotin |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 tests |
| Host name: | Mouse |
| Clone: | J5511 |
| Isotype: | IgG1 |
| Immunogen: | CD16=Derived from the hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells of BALB/c mice immunized with NK lymphocytes |
| Application: | FC |
Description
Description: Identification of human NK cell antigen expressing the 50 -70 kDa M.W. surface antigen associated with the IgG Fc receptor III on NK cells and Neutrophils. CD16 is expressed on approximately 15% of peripheral blood lymphocytes and is present on all resting NK cells. CD16 may be expressed on CD3 T cells from certain individuals.
Additional Text
Purification
Protein A/G Purified
Uniprot ID
Q9ULV2
Antibody Clonality
Monoclonal
Application Notes
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8 C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacture's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Storage Note
Product should be stored at 4-8C. DO NOT FREEZE
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