Mouse anti Human CD25

Catalogue Number: 0251-EXA

Manufacturer:ExAlpha
Type:Monoclonal Primary Antibody - Unconjugated
Alias:CD25*
Shipping Condition:Blue Ice
Unit(s): 100 ug
Host name: Mouse
Clone: ITYV
Isotype: IgG1
Immunogen:
Application: FC

Description

Description: Mouse anti Human CD25 can be used for the identification of human receptor for Interleukin-2 (IL-2R) expressing the 55 kDa surface antigen.

Additional Text

Gene Name

IL2RA

Gene ID

3559

Uniprot ID

P01589

Purification

Protein A/G Purified

Antibody Clonality

Monoclonal

Application Notes

PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Storage Note

Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles