Mouse anti Human CD25, conjugated with PE
Catalogue Number: 0254-NMB
| Manufacturer: | Nordic MuBio |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Alias: | CD25*PE |
| Shipping Condition: | RT |
| Unit(s): | 100 tests |
| Host name: | Mouse |
| Clone: | ITYV |
| Isotype: | IgG1 |
| Immunogen: | CD25=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with PHA-activated human T-lymphocytes. |
| Application: | FC |
Description
Description: Identification of human receptor for Interleukin-2 (IL-2R) expressing the 55,000 M.W. surface antigen.
Additional Text
Gene Name
IL2RA
Gene ID
3559
Uniprot ID
P01589
Purification
Protein A/G Purified
Antibody Clonality
Monoclonal
Application Notes
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Storage Note
Product should be stored at 4-8C. DO NOT FREEZE
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