Mouse anti Human CD27
Catalogue Number: 0271-EXA
| Manufacturer: | ExAlpha |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | CD27 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | DEN-3 |
| Isotype: | IgG1 |
| Immunogen: | |
| Application: | FC |
Description
Description: The CD27 antigen recognizes disulfide-linked dimer nerve growth factor (NGF) super family of cells expressing the 55kD surface antigen. CD27 acts as a co-stimulatory on T lymphocytes with its ligand of CD70.
Additional Text
Gene ID
939
Uniprot ID
P26842
Gene Name
CD27
Purification
Protein A/G Purified
Antibody Clonality
Monoclonal
Application Notes
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Storage Note
Product should be stored at 4-8C. DO NOT FREEZE
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