Mouse anti Human CD28
Catalogue Number: 0281-EXA
| Manufacturer: | ExAlpha |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | CD28 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | B-23 |
| Isotype: | IgG1 |
| Immunogen: | |
| Application: | FC |
Description
Description: Anti-human CD28 binds the 44kDa MW cell surface protein on the surface of most T cells. CD28 acts as the ligand for the B7/BB-1 molecule on the surface of activated B cells. B7/BB-1 costimulates T cells through CD28, along with CD2 and CD3.CD28 antigen is a disulfide-linked homodimeric glycoprotein. The CD28 antigen is present on approximately 60%-80% T lymphocytes (95% of CD4 and 50% of CD8 lymphocytes). CD28 regulates the expression of cytokines by T cells, not only IL-2, but also IL-1 alpha and CSF-1, usually synthesized by accessory cells. CD28 functions as a cell adhesion molecule (CAM) for certain T cell subsets.
Additional Text
Gene ID
940
Gene Name
CD28
Uniprot ID
P10747
Purification
Protein A/G Purified
Antibody Clonality
Monoclonal
Application Notes
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Storage Note
Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles
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