Mouse IgG2a FITC Negative Control
Catalogue Number: 02A2-EXA
| Manufacturer: | ExAlpha |
| Type: | Isotype control - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 tests |
| Host name: | Mouse |
| Clone: | |
| Isotype: | IgG2a |
| Immunogen: | |
| Application: | FC |
Description
Description: Negative control for immunofluorescence staining with FITC conjugated mouse monoclonal antibodies. Assessment of non-specific binding of mouse monoclonal antibodies to human cell surface antigens.
Additional Text
Purification
Protein A/G Purified
Application Notes
Consult the appropriate fact sheet to determine the amount of antibodies to be used as a control for PBMC or Whole Blood. PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Storage Note
Product should be stored at 4-8C. DO NOT FREEZE. Store protected from light. Reagent is stable for the period shown on the vial when stored properly.
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