Green Fluorescent Protein (GFP) (Azide free) (HRP)
Catalogue Number: 030991-HRP-USB
| Manufacturer: | United States Biological |
| Preservative: | Azide-free |
| Physical state: | Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP). |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ul |
| Host name: | Mouse |
| Clone: | 168AT1211 |
| Isotype: | IgG1 |
| Immunogen: | Purified His-tagged GFP protein |
| Application: | ELISA, WB |
Description
Description: Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. Our anti-GFP monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. Our anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by Western Blot.
Additional Text
Caution
FOR RESEARCH USE ONLY
Uniprot ID
P42212
Specificity
Recognizes Green Fluorescent Protein
Purification
Protein G purified
Antibody Clonality
Monoclonal
030991-HRP Datasheet
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