His-Tag (Poly-His, Hexahistidine-HIS, Hexahistidine-Histidine) (AP)
Catalogue Number: 031018-AP-USB
| Manufacturer: | United States Biological |
| Physical state: | Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase. |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 6AT18 |
| Isotype: | IgG1 |
| Immunogen: | Purified recombinant HIS-tagged fusion protein and poly-HIS peptide |
| Application: | ELISA, WB |
Description
Description: Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Our anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.
Additional Text
Specificity
Recognizes His-Tag
Purification
Protein G purified
Antibody Clonality
Monoclonal
031018-AP Datasheet
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