Mouse anti Human CD3 FITC - CD19 PE
Catalogue Number: 0319-NMB
| Manufacturer: | Nordic MuBio |
| Type: | Antibody Packs |
| Alias: | CD3 FITC - CD19 PE |
| Shipping Condition: | RT |
| Unit(s): | 100 tests |
| Host name: | Mouse |
| Clone: | |
| Isotype: | IgG1 |
| Immunogen: | |
| Application: | FC |
Description
Description: The CD3 epitope is expressed on the epsilon chain of the CD3/T cell antigen receptor (TcR) complex. CD3 is present on 65-85% of thymocytes and has a mitogenic effect on peripheral blood T cells. Identification of human T cells expressing the 22-28,000 M.W. surface antigen. Identification of CD19 human B cells associated appoximately 10% of peripheral blood lymphocytes expressing 95,000 M.W. surface antigen
Additional Text
Gene Name
CD3E
Gene ID
916
Uniprot ID
P07766
Purification
Protein A/G Purified
Application Notes
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Storage Note
Product should be stored at 4-8C. DO NOT FREEZE
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations