Mouse anti Human CD33
Catalogue Number: 0331-EXA
| Manufacturer: | ExAlpha |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | CD33 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | FOS |
| Isotype: | IgG1 |
| Immunogen: | CD33=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with FMY9S5 cells containing the CD33 gene. |
| Application: | FC |
Description
Description: Identification of human Monocytes (bright) and Granulocytes (dim) expressing the 67kDa M.W. surface antigen. CD33 is also found on CFU-mix, CFU-GM, CFU-Meg, a portion of BFU-E, myeloblasts, promyelocytes, myelocytes, metamyelocytes but not early precursors
Additional Text
Gene Name
CD33
Gene ID
945
Purification
Protein A/G Purified
Uniprot ID
P20138
Antibody Clonality
Monoclonal
Application Notes
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Storage Note
Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles
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