Mouse anti Human CD4 FITC - CD26 PE

Catalogue Number: 0426-NMB

Manufacturer:Nordic MuBio
Type:Antibody Packs
Alias:CD4 FITC - CD26 PE
Shipping Condition:RT
Unit(s): 100 tests
Host name: Mouse
Clone:
Isotype: IgG1, IgG2a
Immunogen: CD4=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
Application: FC

Description

Description: Identification of CD4 on human helper/inducer T cells expressing the 60,000 M.W. surface antigen (HLA class II reactive). CD4 is present in low density on monocytes. Anti-human CD28 binds the 44kDa MW cell surface protein on the surface of most T cells. The CD26 antigen recognizes the enzyme dipeptidyl peptidase (DPP), a serine protease. Its M.W. is 120 kDa. The CD26 antigen is associated with the binding of the TAT transactivating protein of the human immunodeficiency virus (HIV).

Additional Text

Gene Name

CD4

Gene ID

920

Uniprot ID

P01730

Purification

Protein A/G Purified

Application Notes

PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Storage Note

Product should be stored at 4-8C. DO NOT FREEZE