HMGB1 (High Mobility Group Protein B1) (Biotin)

Catalogue Number: 044885-USB

Manufacturer:	United States Biological
Physical state:	Supplied as a liquid in PBS, 10% glycerol, 0.02% sodium azide.
Type:	Monoclonal Primary Antibody - Conjugated
Alias:	Amphoterin\|high mobility group protein B1\|high-mobility group (nonhistone chromosomal) protein 1\|HMG1\|HMG-1\|HMG3\|SBP-1\|Sulfoglucuronyl carbohydrate binding protein
Shipping Condition:	Blue Ice
Unit(s):	100 ug
Host name:	Human
Clone:	Giby-1-4
Isotype:	IgG2
Immunogen:	Human recombinant HMGB1.
Application:	ELISA, WB

Overview
Datasheets

Description

Description: HMGB1 was originally discovered as an essential DNA-binding protein for regulating p53, NF-kappaB and other important proteins. It is secreted from activated dentric cells, macrophage and nectrotic cells, and acts as a ligand for RAGE, TLR-2 and TLR-4 expressed on surrounding cells. As a result, HMGB1 activates Rac, CDC42 and NF-kappaB inducing localized innate immunity of damaged tissue, tissue regeneration by recruitment of stem cells and hemostasis by induction of tissue factor expression. HMGB1 is also a causative agent of various diseases as it causes localized inflammation such as arteriosclerosis, chronic rheumatoid arthritis and nephritis. Anti-HMGB1, mAb (recombinant) (Giby-1-4) (Biotin) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Additional Text

Caution

FOR RESEARCH USE ONLY

Gene ID

3146

Gene Name

HMGB1

Uniprot ID

P09429

Specificity

Recognizes human, mouse and rat HMGB1.

Purification

Protein A purified

Antibody Clonality

Monoclonal

044885 Datasheet

Price

£516.00

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