Mouse anti Human CD45

Catalogue Number: 0451-EXA

Manufacturer:	ExAlpha
Type:	Monoclonal Primary Antibody - Unconjugated
Alias:	CD45
Shipping Condition:	Blue Ice
Unit(s):	100 ug
Host name:	Mouse
Clone:	BHPT-1
Isotype:	IgG1
Immunogen:	Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with peripheral blood monocytes from rheumatoid arthritis patient
Application:	FC

Overview

Description

Description: Identification of all human leukocytes, lymphocytes, monocytes, polymorphonuclear cells, eosinophils and basophils in peripheral blood expressing the 180-220 kD M. W. T200 family surface antigen. CD45 is also present on thymus, spleen, tonsil and progenitor cells in bone marrow.

Additional Text

Gene Name

PTPRC

Gene ID

5788

Uniprot ID

P08575

Purification

Protein A/G Purified

Antibody Clonality

Monoclonal

Application Notes

PBMC: Add 10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5+: As above, however use only 5 µl per test rather than 10 µl. Excites at 488nm and emits at 670nm. Store protected from light.

Storage Note

Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles

Price

£187.00

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