Mouse anti Human CD45RA
Catalogue Number: 045R1-EXA
| Manufacturer: | ExAlpha |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | CD45RA |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | BHKG-2 |
| Isotype: | IgG1 |
| Immunogen: | Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with peripheral blood monocytes from rheumatoid arthritis patient |
| Application: | FC |
Description
Description: CD45RA recognizes Mr. 220-kdalton of the leucocyte antigen (LCA). CD45RA antigen is a member of the CD45 antigen family that also includes CD45, CD45RO, and CD45RB antigens. CD45RA is expressed on approximately 75% of CD8 lymphocytes and 50% of CD4 lymphocytes, all B cells and NK Cells. CD45RA antigen is expressed on naive T lymphocytes, antigen density decreases upon in vitro activation. A decrease antigen density has been demonstrated for CD4+CD45RA+subset with active multiple sclerosis.
Additional Text
Gene Name
PTPRC
Gene ID
5788
Uniprot ID
P08575
Purification
Protein A/G Purified
Antibody Clonality
Monoclonal
Application Notes
Add 10 µl of MAB / 100 µl of 10^6 PBMC in PBS. Mix Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. Add 10 µl of MAB / 100 µl of Whole Blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse Whole Blood and analyze or fix with 0.5% v/v paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Storage Note
Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles
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