Mouse anti Human CD5 FITC - CD19 PE
Catalogue Number: 0519S-EXA
| Manufacturer: | ExAlpha |
| Type: | Primary Antibody |
| Alias: | CD5 FITC - CD19 PE |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 tests |
| Host name: | Mouse |
| Clone: | |
| Isotype: | IgG1, IgG2a |
| Immunogen: | CD5=Derived from the hybridization of mouse NS-1/Ag4 myeloma cells with spleen cells of BALB/c mice immunized with human t-acute lymphoblastic leukemia (ALL) cells. |
| Application: | FC |
Description
Description: Identification of CD5 human T cells expressing the 67,000 M.W. surface antigen, 85% peripheral blood lymphocytes that form rosettes with sheep red blood cells (E+) and a small subset of B cells. Identification of CD19 PE human B cells associated approximately 10% of peripheral blood lymphocytes expressing the 95,000 M.W. surface antigen.
Additional Text
Gene ID
921
Gene Name
CD5
Uniprot ID
P06127
Purification
Protein A/G Purified
Application Notes
PBMC: Add 10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Storage Note
Product should be stored at 4-8C. DO NOT FREEZE
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