Methyl-CpG Binding Protein 2 (MeCP2)

Catalogue Number: 052088-USB

Manufacturer:	United States Biological
Type:	Monoclonal Primary Antibody - Unconjugated
Shipping Condition:	Blue Ice
Unit(s):	100 ul
Host name:	Mouse
Clone:	5H12
Isotype:	IgG2b
Immunogen:	Full length recombinant protein corresponding to human MeCP2 expressed in E. coli
Application:	ICC, IF, WB, IHC

Overview

Description

Description: Methyl-CpG Binding Protein 2 (MeCP2) is widely expressed in tissues and particularly heavily in neurons. It is a nuclear protein which was found to bind methylated cytosines in CpG islands in DNA, but which may also bind hydroxymethyl cytosine residues (1-3). Methyl and hydroxymethyl cytosines are generally found in regions of DNA regulating the expression of specific genes and MeCP2 is thought to function as a gene regulator, though it may have other functions. The MeCP2 protein contains one methyl-CpG binding domain and two AT hook domains, highly basic peptides which bind AT rich DNA sequences. The MeCP2 gene, MECP2 is located on the X-chromosome and loss of function mutations of the gene cause Rett syndrome, a relatively rare neurodevelopmental, autistic disorder which presents almost exclusively in females, since similar mutation of MECP2 in males is almost invariably lethal (3). Rett patients are apparently normal at birth but develop neurological problems over the period when extensive synaptogenesis is occurring, which coincides with the expression of high levels of MeCP2 in normal brain. The Rett brain shows smaller more densely packed neurons with less extensive dendritic branching (2). Duplication of the MECP2 gene was found to be the cause of a different neurodevelopmental disorder referred to a MECP2 duplication syndrome (4). Levels of MeCP2 normally alter dynamically and the protein is regulated by phosphorylation on multiple sites (5-7).

Additional Text

Gene Name

MECP2

Gene ID

4204

Uniprot ID

P51608

Purification

Purified

Antibody Clonality

Monoclonal

Price

£581.00

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