Catalogue Number: 0563-EXA
| Manufacturer: | ExAlpha |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Alias: | CD56 biotin, NCAM, Neural Cell Adhesion Molecule |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 tests |
| Host name: | Mouse |
| Clone: | C5.9 |
| Isotype: | IgG2b |
| Immunogen: | CD56=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c F1 mice immunized with KG1a cell line. |
| Application: | FC |
Description: CD56, M.W. 150,000, is expressed on approximately 10-25% of human peripheral blood lymphocytes. CD56 (NKH-1) is expressed on human peripheral blood natural killer cells, representing a pan NK-cell antigen. Expressed on non-MHC-restricted cytotoxic T cells.
NCAM1
4684
P13591
Monoclonal
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8 C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Product should be stored at 4-8C. DO NOT FREEZE