Mouse anti Human CD56, conjugated with Biotin

Catalogue Number: 0563-EXA

Manufacturer:ExAlpha
Type:Monoclonal Primary Antibody - Conjugated
Alias:CD56 biotin, NCAM, Neural Cell Adhesion Molecule
Shipping Condition:Blue Ice
Unit(s): 100 tests
Host name: Mouse
Clone: C5.9
Isotype: IgG2b
Immunogen: CD56=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c F1 mice immunized with KG1a cell line.
Application: FC

Description

Description: CD56, M.W. 150,000, is expressed on approximately 10-25% of human peripheral blood lymphocytes. CD56 (NKH-1) is expressed on human peripheral blood natural killer cells, representing a pan NK-cell antigen. Expressed on non-MHC-restricted cytotoxic T cells.

Additional Text

Gene Name

NCAM1

Gene ID

4684

Uniprot ID

P13591

Antibody Clonality

Monoclonal

Application Notes

PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8 C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Storage Note

Product should be stored at 4-8C. DO NOT FREEZE