Mouse anti Human CD61, conjugated with PE
Catalogue Number: 0614-NMB
| Manufacturer: | Nordic MuBio |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | RT |
| Unit(s): | 100 tests |
| Host name: | Mouse |
| Clone: | 43.PL |
| Isotype: | IgG1 |
| Immunogen: | CD61=Derived from the hybridization of mouse X63.Ag8.653 myeloma cells with spleen cells of BALB/c mice immunized with purified platelet membrane glycoproteins. |
| Application: | FC |
Description
Description: CD61 recognizes an Mr 110-kdalton (kDa) protein, also known as gpIIIa, the common subunit (integrin 3-chain) of the gpIIb/IIIa complex and the vitronectin receptor (VNR). The gpIIb/IIIa complex and the VNR are integrins, ie, a/b-heterodimeric glycoprotein complexes that are involved in cell adhesion. With the CD41 antigen (gpIIb or aIIb), the CD61 antigen forms the gpIIb/IIIa complex, which acts as a receptor for fibrinogen, von Willebrand factor (vWf), fibronectin and vitronectin on activated platelets. With the CD51 antigen (VNR a-chain or av), the CD61 antigen forms the VNR, which mediates activation-independent cell adhesion to vitronectin, vWf, fibrinogen, and thrombospondin. The CD61 antigen is found on all normal resting and activated platelets. The CD61 antigen is also found on endothelial cells, megakaryocytes, on some myeloid, erythroid and T-lymphoid leukemic cell lines.
Additional Text
Gene Name
ITGB3
Gene ID
3690
Uniprot ID
P05106
Purification
Protein A/G Purified
Antibody Clonality
Monoclonal
Application Notes
PBMC: Add 5 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 5 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Storage Note
Product should be stored at 4-8C. DO NOT FREEZE
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