Mouse anti Human CD62L, conjugated with PE

Catalogue Number: 0621-NMB

Manufacturer:	Nordic MuBio
Type:	Monoclonal Primary Antibody - Conjugated
Alias:	CD62L
Shipping Condition:	RT
Unit(s):	100 ug
Host name:	Mouse
Clone:	1H3
Isotype:	IgG2a
Immunogen:	CD62L=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with perherial blood T lymphocytes.
Application:	FC

Overview

Description

Description: CD62L is the equivalent of the mouse MEL-14 with a molecular weight of 80 kDa. CD62L is on 60-75% of peripheral blood lymphocytes, Approximately 70-80% of T cells, 10% of thymocytes and most B cells. CD62L reacts with monocytes, granulocytes and a subset of NK cells. The selectins represent a family of adhesion molecules, expressed by leukocytes and endothelial cells that are involved in the regulation of leukocyte traffic. Human leukocyte L selectin is also called LAM-1, LECAM-1, or gp90MEL-14. This antibody to L selectin blocks greater than 95% of lymphocyte adhesion to peripheral lymph node high endothelial venules.

Additional Text

Gene Name

SELL

Gene ID

6402

Uniprot ID

P14151

Purification

Protein A/G Purified

Antibody Clonality

Monoclonal

Application Notes

PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8 C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Storage Note

Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles

Price

£206.00

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