Mouse anti Human CD71, conjugated with PE
Catalogue Number: 0714-NMB
| Manufacturer: | Nordic MuBio |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | RT |
| Unit(s): | 100 tests |
| Host name: | Mouse |
| Clone: | BGX.24 |
| Isotype: | IgG1 |
| Immunogen: | Derived from the hybridization of mouse myeloma cells with spleen cells from BALB/c mice immunized with blast cells from pokeweed mitogen-stimulated peripheral blood cells. |
| Application: | FC |
Description
Description: CD71 is specific for the human transferrin receptor, MW 190 kdaltons (kDa), 90 kDa reduced. CD71 antigen expression is low on normal resting lymphocytes and is expressed on all cells upon activation. The transferrin receptor is essential for iron transport into proliferating cells, such as mitogen-activated and alloantigen-activated lymphoblasts. The transferrin receptor is also present on early erythroid cells but is lost as reticulocytes differentiate into mature erythrocytes.
Additional Text
Gene ID
7037
Gene Name
TFRC
Uniprot ID
P02786
Purification
Ammonium Sulfate Precipitated
Antibody Clonality
Monoclonal
Application Notes
PBMC: Add10 µl of MAB/106 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2o to 8oC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20oC). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. Storage: Antibodies are supplied in PBS, 0.08% sodium azide and 0.2% protein carrier for FITC. Antibodies should be stored at 4-8oC. Monoclonal antibodies should not be frozen. Reagents are stable for the period shown on the vial label when stored properly.
Storage Note
Product should be stored at 4-8C. DO NOT FREEZE
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