Mouse anti Human FAS/APO-1 (CD95), conjugated with PE
Catalogue Number: 0954-NMB
| Manufacturer: | Nordic MuBio |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Alias: | hfas -PE, Anti human fas/Apo1 RPE CD95 |
| Shipping Condition: | RT |
| Unit(s): | 100 tests |
| Host name: | Mouse |
| Clone: | 3.22 |
| Isotype: | IgG1 |
| Immunogen: | Full length CD95 protein |
| Application: | FC, WB |
Description
Description: Activation of either the 55-kD tumor necrosis factor receptor (TNF-R1) or CD95 (Fas/Apo-1) causes apoptosis of cells and liver failure in mice, and has been associated with human liver disorders. The aim of this study was first to clarify the association between CD95 activation, hepatocyte apoptosis, and fulminant liver failure. Next, we investigated whether TNF-R1 and CD95 operate independently of each other in the induction of hepatocyte apoptosis.
Additional Text
Gene Name
FAS
Gene ID
355
Uniprot ID
P25445
Purification
Protein A/G Purified
Antibody Clonality
Monoclonal
Application Notes
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Storage Note
Product should be stored at 4-8C. DO NOT FREEZE
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