Mouse anti Human HLA-DR, conjugated with Biotin
Catalogue Number: 0DR3-EXA
| Manufacturer: | ExAlpha |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Alias: | HLA-DR* Biotin |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 tests |
| Host name: | Mouse |
| Clone: | 423L |
| Isotype: | IgG2a |
| Immunogen: | HLA-DR=Derived from the hybridization of mouse NS-1/1-Ag4 myeloma cells with spleen cells of BALB/c mice immunized with human lymphoblastoid B-cell line RPMI 8866 |
| Application: | FC |
Description
Description: HLA-DR is used for the identification of human B cell and T cell subsets associated with approximately 10% of peripheral blood lymphocytes 28-34,000 M.W. surface antigen, and is present in low density on monocytes and macrophages.
Additional Text
Gene ID
972
Gene Name
CD74
Purification
Protein A/G Purified
Uniprot ID
P04233
Antibody Clonality
Monoclonal
Application Notes
PBMC: Add 10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Storage Note
Product should be stored at 4-8C. DO NOT FREEZE
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