Mouse IgG1 Negative Control
Catalogue Number: 0G11-EXA
| Manufacturer: | ExAlpha |
| Type: | Isotype control - Unconjugated |
| Alias: | IgG1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | ZX3 |
| Isotype: | IgG1 |
| Immunogen: | |
| Application: | FC |
Description
Description: This monoclonal antibody to IgG1 is a negative control for Immunofluorescence staining with Pure, FITC, Biotin, PE and APC mouse monoclonal antibodies of the IgG1 subclass. It is used to monitor the level of non-specific binding of mouse monoclonal antibodies to human cell surface antigens.
Additional Text
Gene Name
IL4
Gene ID
16189
Purification
Protein A/G Purified
Uniprot ID
P07750
Antibody Clonality
Monoclonal
Application Notes
Consult the appropriate fact sheet to determine the amount of antibodies to be used as a control for PBMC or Whole Blood. PBMC: Add 10 µl of MAB/10^6 PBMC in100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of Whole Blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at ?633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Excites at 488nm and emits at 670nm. Store protected from light. Optimal concentration should be evaluated by serial dilutions.
Storage Note
Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles
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