Catalogue Number: 1-1202-5-SAL
Manufacturer: | Salimetrics LLC |
Type: | Human ELISA Kit |
Shipping Condition: | Blue Ice |
Storage Condition: | 4°C |
Unit(s): | 5x96 Tests |
Range: | 10.2 pg/mL - 1000 pg/mL |
Sensitivity: | 5 pg/mL |
Sample type: | Saliva |
Sample size: | 50 µL |
Application: | ELISA |
Description: Salimetrics salivary assay kits are expertly designed, developed and validated to ensure accuracy in saliva and proven to deliver precision results for saliva biomarkers. The Salimetrics Salivary DHEA Enzyme Immunoassay Kit was specifically designed to standardize the detection of DHEA in saliva samples for research and biomedical laboratories. Using a small sample volume, this assay kit has an extended range that spans the expected DHEA levels found in human saliva. The average inter- and intra-assay precision coefficients of variation are low with no deleterious matrix effects often found in saliva which are characterized through dilution- and spike-recovery validation procedures. This DHEA assay kit has also been formatted to minimize cross reactivity for related steroids. DHEA is a major secretory product of the adrenal glands; it is co-released along with cortisol in response to ACTH from the pituitary gland. A major role of DHEA is to act as a circulating precursor of androgens and estrogens in tissues throughout the body, and it has also been associated with immune function. In addition, DHEA is produced directly by the nervous system, where it functions as a neuroactive and neuroprotective factor. Salivary DHEA levels are unaffected by salivary flow rate or salivary enzymes. DHEA exhibits a diurnal rhythm similar to cortisol, with highest levels in the morning after awakening, followed by a decline throughout the afternoon and evening. Kit Contents This 25 pack contains the following components: DHEA EIA Microtitre Break-Apart Plate 25 Plates Assay Diluent 60 mL/Bottle 11 Bottles Wash Buffer Concentrate 10X 100 mL/Bottle 8 Bottles TMB Substrate Solution 25 mL/Bottle 25 Bottles DHEA Standard 1 mL/Vial 10 Vials DHEA High Control 500 μL/Vial 10 Vials DHEA Low Control 500 μL/Vial 10 Vials DHEA Enzyme Conjugate 50 μL/Vial 15 Vials