Monoclonal Antibody to Caspase-3 (Pro and Active) (Clone: ABM1C12)
Catalogue Number: 10-1003-ABO
10-1003 Image
10-1003 Image
10-1003 Image
10-1003 Image
10-1003 Image
10-1003 Image
10-1003 Image
10-1003 Image
10-1003 Image
Fig-3 :Immunohistochemical analysis of Caspase-3 in adenocarcinoma of rectum using Caspase-3 antibody (Clone: ABM1C12) at 5 µg/ml.
10-1003 Image
Fig-5 :Immunohistochemical analysis of Caspase-3 in squamous cell carcinoma of lungs using Caspase-3 antibody (Clone: ABM1C12) at 5 µg/ml.
10-1003 Image
Fig-6 :Immunohistochemical analysis of Caspase-3 in human Small Intestine tissue using Caspase-3 antibody (Clone: ABM1C12) at 10 µg/ml.
10-1003 Image
Fig-7 :Immunohistochemical analysis of Caspase-3 in human Tonsil tissue using Caspase-3 antibody (Clone: ABM1C12) at 10 µg/ml.
10-1003 Image
Fig-8: Intracellular FLOW cytometric analysis of Caspase 3 (Clone : ABM1C12) inon Jurkat cells using 05 µg/10^6 cells of antibody. Goat anti-mouse PE conjugate was used as secondary antibody. Green represents isotope control (ABEOMICS), red represents anti-caspase 3 antibody.
10-1003 Image
Fig 1: Immunohistochemical analysis of Caspase-3 in human colon tissue using Caspase-3 antibody (Clone: ABM1C12) at 5 µg/ml.
10-1003 Image
Fig.2: Western blot analysis of Caspase-3. Anti- Caspase-3 (Clone: ABM1C12) was used at 2 µg/ml on Ramos lysate.
10-1003 Image
Fig-4 :Immunohistochemical analysis of Caspase-3 in human spleen tissue using Caspase-3 antibody (Clone: ABM1C12) at 5 µg/ml.
| Manufacturer: | Abeomics |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Casp3, Cpp32 |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ug, 25 ug |
| Host name: | Mouse |
| Clone: | ABM1C12 |
| Isotype: | IgG1, kappa |
| Immunogen: | Full length recombinant Caspase-3 protein was used as the immunogen for this antibody. |
| Application: | FACS, WB, IHC |
Description
Description: Caspases are a member of the cysteine-aspartic acid protease family. Caspase-3 (31 kDa) is an executionary caspase which directly cleaves and activates poly(ADP-ribose) polymerase (PARP), sterol regulatory element binding proteins (SREBPs) or it can also interact with other caspases like caspase-6, -7 and -9. Increased levels of caspase-3 are involved in Huntington Disease-associated cell death. Caspase-3 is the principal caspase in mediating the cleavage of amyloid-beta 4A precursor protein (APP), which is related with neuronal death in Alzheimer's disease. Like other caspases, caspase-3 is also synthesized as a zymogen procaspase which is activated by specific proteolytic cleavage. High levels of caspase-3 are observed in lung, spleen, heart, liver and kidney, moderate levels in brain and skeletal muscle, and low in testis.
Additional Text
Gene Name
CASP3
Gene ID
836
Purification
Protein G purified
Uniprot ID
P42574
Antibody Clonality
Monoclonal
Application Notes
Western blot analysis: 2-4 µg/ml, Immunohistochemical analysis: 5-10 µg/ml, FACS analysis: 0.5-1 µg/10^6 cells
10-1003 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations