Monoclonal Antibody to Ikk Gamma (Clone: ABM15D9)
Catalogue Number: 10-6017-ABO
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10-6017 Image
10-6017 Image
10-6017 Image
Figure-1: Western blot analysis of Ikk Gamma. Anti-Ikk Gamma antibody (Clone: ABM15D9) was tested at 2 µg/ml on human heart lysate.
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Figure-2: Intracellular flowcytometric analysis of Ikk Gamma in MCF-7 cell line using 0.5 µg/10^6 cells of Anti-Ikk Gamma (Clone: ABM15D9). Green Represent isotype control and red represent Anti-Ikk Gamma (10-6017 Abeomics). Goat Anti-Mouse PE conjugated was used as secondary antibody.
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Figure-3: Intracellular flowcytometric analysis of Ikk Gamma in HeLa cell line using 0.5 µg/10^6 cells of Anti-Ikk Gamma (Clone: ABM15D9). Green Represent isotype control and red represent Anti-Ikk Gamma (10-6017 Abeomics). Goat Anti-Mouse PE conjugated was used as secondary antibody.
| Manufacturer: | Abeomics |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | IKBKG||FIP3||NEMO |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ug, 25 ug |
| Host name: | Mouse |
| Clone: | ABM15D9 |
| Isotype: | IgG1, kappa |
| Immunogen: | A full length recombinant Ikk Gamma protein was used as the immunogen for this antibody. |
| Application: | FACS, WB |
Description
Description: IKK is a trimeric protein complex consisting of two catalytic subunits, IKKalpha and IKKbeta, and a regulatory subunit, IKKgamma. IKKgamma (also termed NEMO or IKBKG) regulates the kinase activity of IKKalpha/beta. IKKgamma plays a critical role in DNA damaged-induced NF-kappaB signaling in a role independent of its function in the IKK complex. While the biological importance of IKKgamma PTMs (Post-Translational Modifications) has been reported under a variety of stimuli, the detection of PTMs has numerous technical limitations. These limitations include both the transient nature of these modifications, as well as low overall abundance of modified IKKgamma inside the cell.
Additional Text
Gene Name
IKBKG
Gene ID
8517
Purification
Protein G purified
Uniprot ID
Q9Y6K9
Antibody Clonality
Monoclonal
Application Notes
Western blot analysis: 2-4 µg/ml, Flowcytometric analysis: 0.5-1 µg/10^6 cells
10-6017 Datasheet
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