Monoclonal Antibody to DNMT3a (Clone: ABM13G4 )
Catalogue Number: 10-7003-ABO
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10-7003 Image
10-7003 Image
Fig-2: Intracellular flow cytometric analysis of Dnmt3a on HEK293 cells using 0.5 µg/10^6 cells of antibody (Clone: ABM13G4). Green represents isotype control; red represents anti-Dnmt3a antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).
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Fig-1: Western blot analysis of DNMT3a. Anti-DNMT3a antibody (Clone: ABM13G4) was used at 2 µg/ml on h heart lysate.
| Manufacturer: | Abeomics |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | DNA (cytosine-5)-methyltransferase 3A, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, M.HsaIIIA |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ug, 25 ug |
| Host name: | Mouse |
| Clone: | ABM13G4 |
| Isotype: | IgG1, kappa |
| Immunogen: | A partial length recombinant protein from DNMT3a was used as the immunogen for this antibody. |
| Application: | FACS, WB |
Description
Description: Dnmt3a belongs to the mammalian methyltransferase gene family which is responsible for tissue-specific gene expression. Dnmt3a together with other methyltransferases conducts de novo methylation of cytosine residues in CpG islands by the enzymatic addition of methyl residues from S-adenosyl-L-methionine to the 5-carbon atom of the cytosine ring. Dnmt3a also directs a metabolic program by repressing key genes to enable the coupling of insulin secretion to glucose levels during beta cell maturation. Loss of Dnmt3a in pancreatic beta cells prevents this developmental metabolic reprogramming, resulting in loss of GSIS (Glucose-Stimulated Insulin Secretion)). Dnmt3a overexpression has been found in several cancers including AML (Acute Myeloid Leukemia).
Additional Text
Gene Name
DNMT3A
Gene ID
1788
Purification
Protein G purified
Uniprot ID
Q9Y6K1
Antibody Clonality
Monoclonal
Application Notes
WB: 2-4 µg/ml, FACS: 0.5-1 µg/10^6
10-7003 Datasheet
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