Monoclonal Antibody to DNMT2 (Clone: ABM1H70)
Catalogue Number: 10-7010-ABO
10-7010 Image
10-7010 Image
10-7010 Image
Fig-2: Intracellular Flow analysis of DNMT2 antibody in Hela cells using 0.5 µg/ 10^6 cells of anti-DNMT2 antibody (ABM1H70). Green represents isotype control; red represents anti-DNMT 2 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).
10-7010 Image
Fig-1: Western blot analysis of DNMT2. Anti-DNMT2 antibody (Clone: ABM1H70) was used at 4 µg/ml on Jurkat and mouse embryonic liver lysates.
| Manufacturer: | Abeomics |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | DNA (cytosine-5)-methyltransferase-like protein 2, DNA methyltransferase homolog HsaIIP, DNMT2 |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ug, 25 ug |
| Host name: | Mouse |
| Clone: | ABM1H70 |
| Isotype: | IgG1, kappa |
| Immunogen: | A partial length recombinant protein from DNMT2 was used as the immunogen for this antibody. |
| Application: | FACS, WB |
Description
Description: DNMT2, also known as tRNA-aspartic acid methyltransferase 1 (TRDMT1), is highly conserved cytosine-C5 methyltransferase that introduces the C38 methylation of tRNAAsp in many species, including lower eukaryotes, plants, insects, and humans. DNMT2 contains all 10 sequence motifs that are conserved among m5C MTases, including the consensus S-adenosyl-L-methionine-binding motifs and the active site ProCys dipeptide. It is a relatively small protein of 391 amino acids and lacks the large N-terminal domains present in the DNMT1 and DNMT3 families. DNMT2 is ubiquitously expressed with multiple mRNA species in most human and mouse adult tissues. It is variably expressed in human cancer cell lines.
Additional Text
Uniprot ID
O14717
Gene Name
TRDMT1
Gene ID
1787
Purification
Protein G purified
Antibody Clonality
Monoclonal
Application Notes
WB: 4-6 µg/ml, FACS: 0.5-1 µg/10^6
10-7010 Datasheet
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