Catalogue Number: 10076-IBL
|Manufacturer:||IBL - (Immuno-Biologicals Laboratories Co Ltd)|
|Type:||Monoclonal Primary Antibody - Unconjugated|
|Shipping Condition:||Blue Ice|
|Immunogen:||A bile canaliculus-rich fraction prepared from rat livers|
Description: Tight junctions function to maintain cellular polarity and permeability barriers in epithelial and endothelial cells. Recently, several component molecules of tight junction have been identified. This monoclonal antibody, 7H6 which reacts with a novel 155 kD tight junction-associated protein in rat livers. The 7H6 antigen is considered as an important molecule for regulation of the barrier function of tight junctions from examination in Madin-Darby canine kidney (MDCK) cells. . Expression of the 7H6 antigen was also examined during rat hepatocarcinogenesis and human cancer tissues. Semiquantitative analysis by confocal laser scanning microscopy revealed that the expression of 7H6 antigen decreased sequentially in preneoplastic lesions and cancer tissues appeared in the liver during hepatocarcinogenesis. The 7H6 antigen was expressed scarcely in HCC with a trabecular pattern, whereas it was expressed intensely at the apical and basolateral membrane of cancer cells with glandular pattern. Examination of human gastric and colon cancer tissues showed that tight junctions were maintained highly in the well-differentiated (gland-forming) adenocarcinomas, but reduced in expression in the poorly-differentiated adenocarcinomas. These indicate that impairment of tight junctions may be associated with malignant phenotypes of cancer cells, such as the loss of cellular polarity and adhesiveness.