Double stranded RNA control Poly(I:C)
Catalogue Number: 10090100-EXA
| Manufacturer: | ExAlpha |
| Type: | Cell Lysate |
| Alias: | Double stranded RNA (dsRNA) control/Poly(I):Poly(C) |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Application: | ELISA, dELISA, DB, inELISA |
Description
Description: The low molecular weight Poly(I:C) dsRNA sequence is intended to be used as a positive control in double-stranded (ds)RNA detection. The sequence does not contain any viral or potentially transforming elements. Positive control for dsRNA detection by anti dsRNA monoclonal J2, J5, K1 and K2 antibodies. Caution: The Poly(I:C) dsRNA positive control, to be combined with our monoclonal anti dsRNA antibodies J2, J5, K1 and K2 in both the direct AN-ELISA and sandwich ELISA, is not intended to be used as a quantitative standard for other dsRNA preparations. The anti dsRNA antibodies may exhibit a different degree of reactivity with different dsRNAs obtained from natural sources. It is therefore only intended to be used as a positive control to see if the ELISA has been executed correctly and that the test shows a linear relationship between the amount of dsRNA and the read out, for example the OD450.
Additional Text
Application Notes
Double stranded RNA control Poly(I:C) is suitable for use as a size standard in dsRNA and RNAi analysis on both non-denaturing polyacrylamide and agarose gels. We suggest to use it as a homogenous, well-characterized positive control for different dsRNA detection methods, e.g. for ELISA and dot-blots. The optimum working dilution of the Poly(I:C) dsRNA control for any specific application should be established by titration.
Storage Note
Store at -20 °C to -80 °C upon arrival, avoid freeze/thaw cycles, store in aliquots.
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