Catalogue Number: 101-006-003-JIR
Manufacturer: | Jackson ImmunoResearch |
Shelf Life: | 12 months |
Preservative: | No preservative |
Physical state: | Liquid |
Type: | Polyclonal Secondary Antibody - Unconjugated |
Shipping Condition: | RT |
Storage Condition: | 2-8°C |
Unit(s): | 1 mg |
Host name: | Goat |
Clone: | |
Isotype: | |
Immunogen: | IgG (H+L) |
Description: F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule bovine IgG. It also reacts with the light chains of other bovine immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
AffiniPure
Polyclonal
Goat Anti-Bovine IgG (H+L) unconjugated secondary antibody. This is an affinity purified F(ab′)2 Fragment polyclonal antibody.
10-20 µg / ml Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Storage: Store at 2-8°C under sterile conditions. Prepare working dilution on day of use. Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.