iFluor® 488 Tetrazine
Catalogue Number: 1014-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Fluorescent Reagents |
| Shipping Condition: | RT |
| Unit(s): | 1 mg |
Description
Description: The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. iFluor® 488 tetrazine can be readily used to label TCO-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. The conjugates prepared with iFluor® 488 dyes are far superior to those of fluorescein derivatives such as FITC. iFluor® 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor® 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor® 488 has spectral properties similar to Alexa Fluor® 488. In some cases, it demonstrates brighter signals (Alexa Fluor® is the trademark of Invitrogen).
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
1014 Protocol
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