p21WAF1 Anti-Human Monoclonal [Clone WA-1]

Catalogue Number: 1026-MSM1-P1-EQB

Manufacturer:Enquire Bioreagents
Shelf Life:2 years
Preservative:BSA and Azide
Physical state:Liquid
Type:Monoclonal Primary Antibody
Shipping Condition:Blue Ice
Unit(s): 100 ug
Host name: Mouse
Clone: WA-1
Isotype: IgG1, kappa
Immunogen: Human recombinant p21 protein

Additional Text

Molecular Weight

21kDa

Hu-Chromosome Location

6p21.31

Gene Symbol

CDKN1A

Concentration

200ug/ml

Additional application information

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.

Specificity

This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). In ELISA, MAb WA-1 is useful either as a solid phase or for detection of p21 protein.