iFluor® 690 succinimidyl ester
Catalogue Number: 1042-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Fluorescent Reagents |
| Shipping Condition: | RT |
| Unit(s): | 1 mg |
Description
Description: AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, 633, and 647 nm) or other excitation sources. iFluor® 690 dyes have fluorescence excitation and emission maxima of ~685 nm and ~704 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 700 labeling dye (Alexa Fluor® is the trademark of Invitrogen). iFluor® 690 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. Our preliminary tests indicated that it has superior FRET when conjugated to PE, making it an excellent acceptor dye for preparing tandems with APC, PE, and PerCP. iFluor® 690-based tandems provide unique colors that are extremely useful for spectral flow cytometry applications.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
1042 Protocol
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