iFluor® 488 hydrazide

Catalogue Number: 1082-AAT

Manufacturer:AAT Bioquest
Shelf Life:12 months
Type:Fluorescent Reagents
Shipping Condition:RT
Unit(s): 1 mg

Description

Description: Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor™ 488 dyes (Ex/Em=491 nm/520 nm) are far superior compared to conjugates of fluorescein derivatives such as FITC. iFluor™ 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor™ 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor™ 488 hydrazide is reasonably stable and shows good reactivity and selectivity with carbonyl group (such as aldehyde). This iFluor™ 488 has spectral properties and reactivity similar to Alexa Fluor® 488 hydrazide ( Alexa Fluor® is the trademark of Invitrogen).

Additional Text

Storage Note

Freeze (< -15 °C); Minimize light exposure;|12 months