Catalogue Number: 109-006-098-JIR
|Shelf Life:||12 months|
|Type:||Polyclonal Secondary Antibody - Unconjugated|
|Immunogen:||IgG, Fcγ fragment|
Description: F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG. No antibody was detected against human IgM or IgA, or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins, but it may cross-react with immunoglobulins from other species.
Goat Anti-Human IgG, Fcγ fragment unconjugated secondary antibody. This is an affinity purified F(ab′)2 Fragment polyclonal antibody and has been tested and/or adsorbed to ensure minimal cross reactivity with Bovine, Horse and Mouse serum proteins.
10-20 µg / ml Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Storage: Store at 2-8°C under sterile conditions. Prepare working dilution on day of use. Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.