Biotin-SP-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Human IgG, Fcγ Fragment Specific (min X Bov,Hrs,Ms Sr Prot)

Catalogue Number: 109-066-098-JIR

Manufacturer:Jackson ImmunoResearch
Shelf Life:12 months
Preservative:0.05% Sodium azide
Physical state:Freeze-dried solid
Type:Polyclonal Secondary Antibody - Conjugated
Shipping Condition:RT
Unit(s): 0.5 ml
Host name: Goat
Clone:
Isotype: IgG
Immunogen: IgG, Fcγ fragment
Application: ELISA, FC, ICC, WB, IHC

Description

Description: F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG. No antibody was detected against human IgM or IgA, or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins, but it may cross-react with immunoglobulins from other species.

Additional Text

Purification

AffiniPure

Antibody Clonality

Polyclonal

Short Description

Goat Anti-Human IgG, Fcγ fragment secondary antibody, conjugated to Biotin-SP (long spacer). This is an affinity purified F(ab′)2 Fragment polyclonal antibody and has been tested and/or adsorbed to ensure minimal cross reactivity with Bovine, Horse and Mouse serum proteins.

Application Notes

1:20,000 - 1:400,000 for ELISA and Western blotting using enzyme-conjugated streptavidin 1:500 - 1:5,000 for enzyme immunohisto/cytochemistry 1:200 - 1:1,000 for flow cytometry and fluorescence immunohisto/cytochemistry Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Storage Note

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.