R-Phycoerythrin-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Human IgG, F(ab')2 Fragment Specific (min X Bov,Hrs,Ms Sr Prot)

Catalogue Number: 109-116-097-JIR

Manufacturer:	Jackson ImmunoResearch
Shelf Life:	6 months
Preservative:	0.05% Sodium azide
Physical state:	Freeze-dried solid
Type:	Polyclonal Secondary Antibody - Conjugated
Shipping Condition:	RT
Unit(s):	1 ml
Host name:	Goat
Clone:
Isotype:
Immunogen:	IgG, F(ab')2 fragment

Overview
Datasheets

Description

Description: F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the F(ab')2/Fab portion of human IgG. It also reacts with the light chains of other human immunoglobulins. No antibody was detected against the Fc portion of human IgG or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins, but it may cross-react with immunoglobulins from other species.

Additional Text

Purification

AffiniPure

Antibody Clonality

Polyclonal

Short Description

Goat Anti-Human IgG, F(ab′)2 fragment secondary antibody, conjugated to R-Phycoerythrin . This is an affinity purified F(ab′)2 Fragment polyclonal antibody and has been tested and/or adsorbed to ensure minimal cross reactivity with Bovine, Horse and Mouse serum proteins.

Application Notes

1:50 - 1:200 for most applications Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Storage Note

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Store at 2-8°C - do not freeze. Prepare working dilution on day of use. Expiration date: six months from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Product Specification Sheets