Catalogue Number: 109-185-043-JIR
Manufacturer: | Jackson ImmunoResearch |
Shelf Life: | 12 months |
Preservative: | 0.05% Sodium azide |
Physical state: | Freeze-dried solid |
Type: | Polyclonal Secondary Antibody - Conjugated |
Shipping Condition: | RT |
Unit(s): | 1 ml |
Host name: | Goat |
Clone: | |
Isotype: | |
Immunogen: | IgM, Fc5µ fragment |
Application: | ICC, IHC |
Description: Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc5µ portion of the human IgM heavy chain but not with human IgG, IgA, or the light chains of human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with IgM from other species.
AffiniPure
Polyclonal
Goat Anti-Human IgM, Fc5μ fragment secondary antibody, conjugated to 4 nm Colloidal Gold. This is an affinity purified whole IgG polyclonal antibody.
Histo-/Cyto-Chemistry:- 1:20-1:200 Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Extended Storage after Rehydration: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.