Alexa Fluor® 647-conjugated AffiniPure® Fab Fragment Goat Anti-Human IgM, Fc5µ Fragment Specific

Catalogue Number: 109-607-043-JIR

Manufacturer:	Jackson ImmunoResearch
Shelf Life:	12 months
Preservative:	0.05% Sodium azide
Physical state:	Freeze-dried solid
Type:	Polyclonal Secondary Antibody - Conjugated
Shipping Condition:	RT
Unit(s):	0.5 mg
Host name:	Goat
Clone:
Isotype:
Immunogen:	IgM, Fc5µ fragment
Application:	Lbl

Overview
Datasheets

Description

Description: Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa. Based on antigen-binding assay and/or ELISA, the antibody reacts with the Fc5µ portion of the human IgM heavy chain but not with human IgG, IgA, or the light chains of human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with IgM from other species.

Additional Text

Purification

AffiniPure

Antibody Clonality

Polyclonal

Short Description

Goat Anti-Human IgM, Fc5μ fragment secondary antibody, conjugated to Alexa Fluor® 647. This is an affinity purified Fab Fragment polyclonal antibody.

Application Notes

1:100 - 1:800 for sequential labeling applications. To complex with primary antibody in solution, use 1:1 weight ratio of Fab:primary antibody (15:1 molar ratio). Vortex and incubate for 30 minutes at room temperature prior to use. Titrate complex to optimal dilution for assay. Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.