Polyclonal Antibody to Beta actin
Catalogue Number: 11-13012-ABO
11-13012 Image
11-13012 Image
11-13012 Image
11-13012 Image
Figure-1: Western blot analysis of Beta actin. Anti- Beta actin antibody (11-13012) was used at 4 µg/ml on 1) Raw, 2) 3T3, 3) Hela and 4) U87 lysates.
11-13012 Image
Figure-2: Western blot analysis of Beta actin. Anti- Beta actin antibody (11-13012) was used at 1 µg/ml on (1) Miapica-2, (2) HepG2, (3) Jurkat, (4) HCT-116, (5) 293, (6) THP1, (7) A549, (8) C2C12 and (9) MCF-7 lysates.
11-13012 Image
Figure-3: Western blot analysis of Beta actin. Anti- Beta actin antibody (11-13012) was used at 2 µg/ml on (1) Rat Spleen, (2) Human Intestine, (3) Mouse Spleen and (4) BV2 lysates.
| Manufacturer: | Abeomics |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Actin, cytoplasmic 1, ACTB |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ug, 25 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | A partial length recombinant Beta actin protein (amino acids 1-200) was used as the immunogen for this antibody. |
| Application: | WB |
Description
Description: B-actin (ACTB) has traditionally been regarded as an endogenous housekeeping gene and has been widely used as a reference gene/protein in quantifying expression levels in tumors. It supports fundamental cellular processes in healthy and diseased cells including cell adhesion, migration, cytokinesis and maintenance of cell polarity. However, B-actin is closely associated with a variety of cancers and accumulating evidence indicates that B-actin is de-regulated in liver, melanoma, renal, colorectal, gastric, pancreatic, esophageal, lung, breast, prostate, ovarian cancers, leukemia and lymphoma. This protein is generally found to be up-regulated in the majority of tumor cells and tissues.
Additional Text
Gene Name
ACTB
Uniprot ID
P60709
Gene ID
60
Purification
Protein A purified
Antibody Clonality
Polyclonal
Application Notes
Western blot analysis: 4-6 µg/ml:
11-13012 Datasheet
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