ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
Catalogue Number: 11003-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Enzyme Substrates |
| Shipping Condition: | RT |
| Unit(s): | 1 L |
Description
Description: Horseradish peroxidase (HRP) and HRP conjugates facilitate the ABTS oxidation in the presence of hydrogen peroxide, turning ABTS into its blue-green oxidized product. ReadiUse™ TMB Substrate Solution is a premixed solution of TMB substrate with hydrogen peroxide. It produces a blue product upon interaction with HRP or HRP conjugates without the addition of hydrogen peroxide. The soluble blue product can be quantitated at 650 nm. Use of a stop solution enhances sensitivity 2-4 fold and the resulting yellow solution can be read at 450 nm. ReadiUse™ TMB Substrate Solution provides an convenient and ultrasensitive quantitative substrate system.
Additional Text
Storage Note
Refrigerated (2-8 °C); Minimize light exposure;|12 months
11003 Protocol
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