Amplite® Red
Catalogue Number: 11011-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Fluorophores |
| Shipping Condition: | RT |
| Unit(s): | 1000 Assays |
Description
Description: Our Amplite™ Red is a sensitive fluorogenic peroxidase substrate that generates a highly red fluorescent product that has maximum absorption of 571 nm and maximum emission of 585 nm. Unlike other HRP substrates such as dihydrofluoresceins and dihydrorhodamines, the air-oxidation of Amplite™ Red is minimal. Amplite™ Red is one of the most sensitive and stable fluorogenic probes for detecting HRP and H2O2. Amplite™ Red has been widely used to detect HRP in many immunoassays. On the other hand, Amplite™ Red can also be used to detect trace amount of H2O2. The Amplite™ Red-based H2O2 detection is at least one order of magnitude more sensitive than the commonly used scopoletin assay for H2O2. Because H2O2 is produced in many enzymatic redox reactions, Amplite™ Red can be used in coupled enzymatic reactions to detect the activity of many oxidases and/or related enzymes/substrates or cofactors such as glucose, acetylcholine and cholesterol, L-glutamate, amino acids, etc.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure; Desiccated;|12 months
11011 Protocol
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