ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*

Catalogue Number: 11012-AAT

Manufacturer:AAT Bioquest
Shelf Life:12 months
Type:Enzyme Substrates
Shipping Condition:RT
Unit(s): 100 ml

Description

Description: Horseradish peroxidase (HRP) and HRP conjugates facilitate the ABTS oxidation in the presence of hydrogen peroxide, turning ABTS into its blue-green oxidized product. ReadiUse™ TMB Substrate Solution is a premixed solution of TMB substrate with hydrogen peroxide. It produces a blue product upon interaction with HRP or HRP conjugates without the addition of hydrogen peroxide. The soluble blue product can be quantitated at 650 nm. Use of a stop solution enhances sensitivity 2-4 fold and the resulting yellow solution can be read at 450 nm. ReadiUse™ TMB Substrate Solution provides an convenient and ultrasensitive quantitative substrate system.

Additional Text

Storage Note

Refrigerated (2-8 °C); Minimize light exposure;|12 months