Signal Guard™ HRP reaction stopping reagent
Catalogue Number: 11020-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Immunodetection Kits and Reagents |
| Shipping Condition: | RT |
| Unit(s): | 500 tests |
Description
Description: HRP coupling reactions provide sensitive biomolecular assays based on hydrogen peroxide–generating enzyme systems linked to peroxidase–mediated oxidation. Fluorogenic HRP substrates are preferred to use for enhancing assay sensitivities. Among them, the most commonly used HRP substrates include ADHP (also called Amplex® Red, #11000), Amplex® UltraRed and Amplite™ Red. Typically, detection reactions are performe in microplate wells and are initiated by adding a fluorogenic HRP substrate, resulting in a continuous fluorescence increase. It is critical to ensure that the timing of the standard and unknown sample measurements is the same. Our Signal Guard™ HRP reaction stopping solution provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable. The Signal Guard™ HRP reaction stopping solution is designed for use in conjunction with ADHP (Amplex® Red ), Amplite™ and Amplex®UltraRed fluorogenic substrates. It can also be used in other HRP reaction systems.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
11020 Protocol
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