Amplite® Fluorimetric Glutamate Oxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Glutamate Oxidase Assay Kit Red Fluorescence

Catalogue Number: 11302-AAT

Manufacturer:	AAT Bioquest
Type:	Enzyme Assays
Shipping Condition:	Blue Ice
Unit(s):	200 Tests

Overview
Datasheets

Description

Description: Glutamate oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-NH2 group of donors with oxygen as an acceptor. It is an enzyme that specifically catalyzes the oxidative deamination of L-glutamate in the presence of water and oxygen with the formation of o-ketoglutarate, ammonia, and hydrogen peroxide. The Amplite® Fluorimetric Glutamate Oxidase Assay Kit provides a quick and ultrasensitive method for the measurement of glutamate oxidase in solution and in cell lysates. In the assay, L-glutamic acid is oxidized to µ-ketoglutarate, NH3 and H2O2 by glutamate oxidase. L-Alanine and L-glutamate-pyruvate transaminase are included in the reaction, resulting in multiple cycles of the initial reaction, thus significantly amplifying the production of H2O2. The kit uses our Amplite® Red substrate which enables a dual recordable mode. The fluorescent signal can be easily read by either a fluorescence microplate reader or an absorbance microplate reader. With the Amplite® Glutamate Oxidase Assay kit, we have detected as little as 40 uU/mL glutamate oxidase in a 100 µL reaction volume. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.

Additional Text

Storage Note

Multiple;|6 months

11302 MSDS

11302 Protocol

Price

£228.00

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